Department Of
Chemical Engineering (Ch.E)
UV-VIS Spectroscopy

Overview

<p><span data-huuid="3744881301278352256">UV-Vis spectroscopy is&nbsp;<mark class="QVRyCf">an analytical technique that measures the amount of ultraviolet (UV) or visible light a chemical compound absorbs</mark>.&nbsp;</span><span data-huuid="3744881301278353507">By analyzing the specific wavelengths of light absorbed, it can identify and quantify substances based on their interaction with light.&nbsp;</span><span data-huuid="3744881301278354758">This is possible because different compounds absorb light at different wavelengths, which is related to the energy required to promote electrons to a higher energy state.&nbsp;</span></p><br/><p class="MsoNormal" style="text-align: justify;">Applications:</p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Quantitative Analysis:&nbsp;</strong>The intensity of the absorbed light is directly related to the concentration of the substance, making it useful for determining how much of a compound is present.&nbsp;</p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Chemical Identification:&nbsp;</strong>The unique absorption spectrum can help identify unknown compounds.&nbsp;</p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Quality Control:&nbsp;</strong>It is widely used in industries like pharmaceuticals and food to ensure product quality, purity, and safety.&nbsp;</p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Non-destructive Analysis:&nbsp;</strong>The technique is non-destructive, meaning samples are not damaged and can be studied repeatedly.&nbsp;</p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Research:&nbsp;</strong>It's a fundamental tool in research for studying the properties of molecules and tracking chemical reactions.&nbsp;</p>