Department Of
Chemical Engineering (Ch.E)
UV-VIS Spectroscopy
Overview
<p><span data-huuid="3744881301278352256">UV-Vis spectroscopy is <mark class="QVRyCf">an analytical technique that measures the amount of ultraviolet (UV) or visible light a chemical compound absorbs</mark>. </span><span data-huuid="3744881301278353507">By analyzing the specific wavelengths of light absorbed, it can identify and quantify substances based on their interaction with light. </span><span data-huuid="3744881301278354758">This is possible because different compounds absorb light at different wavelengths, which is related to the energy required to promote electrons to a higher energy state. </span></p><br/><p class="MsoNormal" style="text-align: justify;">Applications:</p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Quantitative Analysis: </strong>The intensity of the absorbed light is directly related to the concentration of the substance, making it useful for determining how much of a compound is present. </p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Chemical Identification: </strong>The unique absorption spectrum can help identify unknown compounds. </p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Quality Control: </strong>It is widely used in industries like pharmaceuticals and food to ensure product quality, purity, and safety. </p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Non-destructive Analysis: </strong>The technique is non-destructive, meaning samples are not damaged and can be studied repeatedly. </p><br/><p class="MsoNormal" style="text-align: justify;"><strong>Research: </strong>It's a fundamental tool in research for studying the properties of molecules and tracking chemical reactions. </p>
